plasmid sequencing services Search Results


the validated pu6-bbsi-chirna plasmid containing the clamp target sequence  (Genetic Services Inc)
90
Genetic Services Inc the validated pu6-bbsi-chirna plasmid containing the clamp target sequence
The Validated Pu6 Bbsi Chirna Plasmid Containing The Clamp Target Sequence, supplied by Genetic Services Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+sequencing+services/bio_rxiv__042820-150-13-3?v=Genetic+Services+Inc
Average 90 stars, based on 1 article reviews
the validated pu6-bbsi-chirna plasmid containing the clamp target sequence - by Bioz Stars, 2026-07
90/100 stars
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puast-derived plasmids mini white gene) containing gfp-smo coding sequences  (Genetic Services Inc)
90
Genetic Services Inc puast-derived plasmids mini white gene) containing gfp-smo coding sequences
Smo phosphorylation site variants show transdominant effects in vitro and in vivo. (A) Overexpressed untagged and GFP-tagged Smo variants cause transdominant effects on reporter activity. Either pAcSV- or <t>pUAST-based</t> plasmids expressing the Smo variants shown were transfected in the presence of endogenous Smo. The results of a representative ptc-Luc reporter assay are shown. (B) Overexpression of GFP-tagged Smo-Glu in embryos leads to expansion of Wg expression. Immunofluorescence using anti-GFP (green) and anti-Wg (red) Abs is shown for dorsal views at ×10 and ×25 of embryos at extended germ-band stage that are either wild-type (WT; w1118) or expressing UAS GFP-tagged wild-type (Smo), III,V,VI Ala (Ala), or III,V,VI Glu (Glu) forms of Smo using <t>the</t> <t>prd-GAL4</t> driver. P ↔ A, orientation of the posterior/anterior axis. (C) Overexpression of Smo variants leads to wing patterning alterations. Wings collected from adult flies that are either wild-type (WT) or heterozygous for the ptc-GAL4 (Upper) or 71B GAL4 (Lower) driver and expressing GFP-Smo variants as in B are shown. Longitudinal veins 1–5 are labeled. Asterisk, proximal L3 and L4 fusion for the GFP-Smo-Ala variant overexpressed with ptc-GAL4; arrowheads, ectopic veination near the proximal end of L3 when GFP-Smo is overexpressed (black) and proximal L3 and L4 fusion when GFP-Smo-Ala is overexpressed (white) using 71B GAL4. Flies expressing GFP-Smo-Glu die as pupae with ptc-GAL4 and as pharate adults with 71B GAL4. A severely defective wing from an escaper from the GFP-Smo-Glu 71B GAL4 line is shown (Lower Right).
Puast Derived Plasmids Mini White Gene) Containing Gfp Smo Coding Sequences, supplied by Genetic Services Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+sequencing+services/pmc00535705-118-13-32?v=Genetic+Services+Inc
Average 90 stars, based on 1 article reviews
puast-derived plasmids mini white gene) containing gfp-smo coding sequences - by Bioz Stars, 2026-07
90/100 stars
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plasmid dna sequencing  (AgriGenome Labs)
90
AgriGenome Labs plasmid dna sequencing
Smo phosphorylation site variants show transdominant effects in vitro and in vivo. (A) Overexpressed untagged and GFP-tagged Smo variants cause transdominant effects on reporter activity. Either pAcSV- or <t>pUAST-based</t> plasmids expressing the Smo variants shown were transfected in the presence of endogenous Smo. The results of a representative ptc-Luc reporter assay are shown. (B) Overexpression of GFP-tagged Smo-Glu in embryos leads to expansion of Wg expression. Immunofluorescence using anti-GFP (green) and anti-Wg (red) Abs is shown for dorsal views at ×10 and ×25 of embryos at extended germ-band stage that are either wild-type (WT; w1118) or expressing UAS GFP-tagged wild-type (Smo), III,V,VI Ala (Ala), or III,V,VI Glu (Glu) forms of Smo using <t>the</t> <t>prd-GAL4</t> driver. P ↔ A, orientation of the posterior/anterior axis. (C) Overexpression of Smo variants leads to wing patterning alterations. Wings collected from adult flies that are either wild-type (WT) or heterozygous for the ptc-GAL4 (Upper) or 71B GAL4 (Lower) driver and expressing GFP-Smo variants as in B are shown. Longitudinal veins 1–5 are labeled. Asterisk, proximal L3 and L4 fusion for the GFP-Smo-Ala variant overexpressed with ptc-GAL4; arrowheads, ectopic veination near the proximal end of L3 when GFP-Smo is overexpressed (black) and proximal L3 and L4 fusion when GFP-Smo-Ala is overexpressed (white) using 71B GAL4. Flies expressing GFP-Smo-Glu die as pupae with ptc-GAL4 and as pharate adults with 71B GAL4. A severely defective wing from an escaper from the GFP-Smo-Glu 71B GAL4 line is shown (Lower Right).
Plasmid Dna Sequencing, supplied by AgriGenome Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+sequencing+services/pm37019052-78-11-14?v=AgriGenome+Labs
Average 90 stars, based on 1 article reviews
plasmid dna sequencing - by Bioz Stars, 2026-07
90/100 stars
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plasmid sequences services  (Plasmidsaurus)
86
Plasmidsaurus plasmid sequences services
Smo phosphorylation site variants show transdominant effects in vitro and in vivo. (A) Overexpressed untagged and GFP-tagged Smo variants cause transdominant effects on reporter activity. Either pAcSV- or <t>pUAST-based</t> plasmids expressing the Smo variants shown were transfected in the presence of endogenous Smo. The results of a representative ptc-Luc reporter assay are shown. (B) Overexpression of GFP-tagged Smo-Glu in embryos leads to expansion of Wg expression. Immunofluorescence using anti-GFP (green) and anti-Wg (red) Abs is shown for dorsal views at ×10 and ×25 of embryos at extended germ-band stage that are either wild-type (WT; w1118) or expressing UAS GFP-tagged wild-type (Smo), III,V,VI Ala (Ala), or III,V,VI Glu (Glu) forms of Smo using <t>the</t> <t>prd-GAL4</t> driver. P ↔ A, orientation of the posterior/anterior axis. (C) Overexpression of Smo variants leads to wing patterning alterations. Wings collected from adult flies that are either wild-type (WT) or heterozygous for the ptc-GAL4 (Upper) or 71B GAL4 (Lower) driver and expressing GFP-Smo variants as in B are shown. Longitudinal veins 1–5 are labeled. Asterisk, proximal L3 and L4 fusion for the GFP-Smo-Ala variant overexpressed with ptc-GAL4; arrowheads, ectopic veination near the proximal end of L3 when GFP-Smo is overexpressed (black) and proximal L3 and L4 fusion when GFP-Smo-Ala is overexpressed (white) using 71B GAL4. Flies expressing GFP-Smo-Glu die as pupae with ptc-GAL4 and as pharate adults with 71B GAL4. A severely defective wing from an escaper from the GFP-Smo-Glu 71B GAL4 line is shown (Lower Right).
Plasmid Sequences Services, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+sequencing+services/pmc12684380-567-7-12?v=Plasmidsaurus
Average 86 stars, based on 1 article reviews
plasmid sequences services - by Bioz Stars, 2026-07
86/100 stars
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high copy whole plasmid sequencing service  (Plasmidsaurus)
86
Plasmidsaurus high copy whole plasmid sequencing service
Smo phosphorylation site variants show transdominant effects in vitro and in vivo. (A) Overexpressed untagged and GFP-tagged Smo variants cause transdominant effects on reporter activity. Either pAcSV- or <t>pUAST-based</t> plasmids expressing the Smo variants shown were transfected in the presence of endogenous Smo. The results of a representative ptc-Luc reporter assay are shown. (B) Overexpression of GFP-tagged Smo-Glu in embryos leads to expansion of Wg expression. Immunofluorescence using anti-GFP (green) and anti-Wg (red) Abs is shown for dorsal views at ×10 and ×25 of embryos at extended germ-band stage that are either wild-type (WT; w1118) or expressing UAS GFP-tagged wild-type (Smo), III,V,VI Ala (Ala), or III,V,VI Glu (Glu) forms of Smo using <t>the</t> <t>prd-GAL4</t> driver. P ↔ A, orientation of the posterior/anterior axis. (C) Overexpression of Smo variants leads to wing patterning alterations. Wings collected from adult flies that are either wild-type (WT) or heterozygous for the ptc-GAL4 (Upper) or 71B GAL4 (Lower) driver and expressing GFP-Smo variants as in B are shown. Longitudinal veins 1–5 are labeled. Asterisk, proximal L3 and L4 fusion for the GFP-Smo-Ala variant overexpressed with ptc-GAL4; arrowheads, ectopic veination near the proximal end of L3 when GFP-Smo is overexpressed (black) and proximal L3 and L4 fusion when GFP-Smo-Ala is overexpressed (white) using 71B GAL4. Flies expressing GFP-Smo-Glu die as pupae with ptc-GAL4 and as pharate adults with 71B GAL4. A severely defective wing from an escaper from the GFP-Smo-Glu 71B GAL4 line is shown (Lower Right).
High Copy Whole Plasmid Sequencing Service, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+sequencing+services/bio_rxiv__2025__11__25__690463-154-7-5?v=Plasmidsaurus
Average 86 stars, based on 1 article reviews
high copy whole plasmid sequencing service - by Bioz Stars, 2026-07
86/100 stars
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complete plasmid sequencing service  (Eurofins)
86
Eurofins complete plasmid sequencing service
Smo phosphorylation site variants show transdominant effects in vitro and in vivo. (A) Overexpressed untagged and GFP-tagged Smo variants cause transdominant effects on reporter activity. Either pAcSV- or <t>pUAST-based</t> plasmids expressing the Smo variants shown were transfected in the presence of endogenous Smo. The results of a representative ptc-Luc reporter assay are shown. (B) Overexpression of GFP-tagged Smo-Glu in embryos leads to expansion of Wg expression. Immunofluorescence using anti-GFP (green) and anti-Wg (red) Abs is shown for dorsal views at ×10 and ×25 of embryos at extended germ-band stage that are either wild-type (WT; w1118) or expressing UAS GFP-tagged wild-type (Smo), III,V,VI Ala (Ala), or III,V,VI Glu (Glu) forms of Smo using <t>the</t> <t>prd-GAL4</t> driver. P ↔ A, orientation of the posterior/anterior axis. (C) Overexpression of Smo variants leads to wing patterning alterations. Wings collected from adult flies that are either wild-type (WT) or heterozygous for the ptc-GAL4 (Upper) or 71B GAL4 (Lower) driver and expressing GFP-Smo variants as in B are shown. Longitudinal veins 1–5 are labeled. Asterisk, proximal L3 and L4 fusion for the GFP-Smo-Ala variant overexpressed with ptc-GAL4; arrowheads, ectopic veination near the proximal end of L3 when GFP-Smo is overexpressed (black) and proximal L3 and L4 fusion when GFP-Smo-Ala is overexpressed (white) using 71B GAL4. Flies expressing GFP-Smo-Glu die as pupae with ptc-GAL4 and as pharate adults with 71B GAL4. A severely defective wing from an escaper from the GFP-Smo-Glu 71B GAL4 line is shown (Lower Right).
Complete Plasmid Sequencing Service, supplied by Eurofins, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+sequencing+services/pm42042791-165-7-6?v=Eurofins
Average 86 stars, based on 1 article reviews
complete plasmid sequencing service - by Bioz Stars, 2026-07
86/100 stars
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geneart service plasmid sequence verification  (Thermo Fisher)
N/A
The GeneArt Gene Synthesis service offers chemical synthesis cloning and sequence verification of virtually any desired genetic sequence
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Image Search Results


Smo phosphorylation site variants show transdominant effects in vitro and in vivo. (A) Overexpressed untagged and GFP-tagged Smo variants cause transdominant effects on reporter activity. Either pAcSV- or pUAST-based plasmids expressing the Smo variants shown were transfected in the presence of endogenous Smo. The results of a representative ptc-Luc reporter assay are shown. (B) Overexpression of GFP-tagged Smo-Glu in embryos leads to expansion of Wg expression. Immunofluorescence using anti-GFP (green) and anti-Wg (red) Abs is shown for dorsal views at ×10 and ×25 of embryos at extended germ-band stage that are either wild-type (WT; w1118) or expressing UAS GFP-tagged wild-type (Smo), III,V,VI Ala (Ala), or III,V,VI Glu (Glu) forms of Smo using the prd-GAL4 driver. P ↔ A, orientation of the posterior/anterior axis. (C) Overexpression of Smo variants leads to wing patterning alterations. Wings collected from adult flies that are either wild-type (WT) or heterozygous for the ptc-GAL4 (Upper) or 71B GAL4 (Lower) driver and expressing GFP-Smo variants as in B are shown. Longitudinal veins 1–5 are labeled. Asterisk, proximal L3 and L4 fusion for the GFP-Smo-Ala variant overexpressed with ptc-GAL4; arrowheads, ectopic veination near the proximal end of L3 when GFP-Smo is overexpressed (black) and proximal L3 and L4 fusion when GFP-Smo-Ala is overexpressed (white) using 71B GAL4. Flies expressing GFP-Smo-Glu die as pupae with ptc-GAL4 and as pharate adults with 71B GAL4. A severely defective wing from an escaper from the GFP-Smo-Glu 71B GAL4 line is shown (Lower Right).

Journal:

Article Title: Extensive phosphorylation of Smoothened in Hedgehog pathway activation

doi: 10.1073/pnas.0408093101

Figure Lengend Snippet: Smo phosphorylation site variants show transdominant effects in vitro and in vivo. (A) Overexpressed untagged and GFP-tagged Smo variants cause transdominant effects on reporter activity. Either pAcSV- or pUAST-based plasmids expressing the Smo variants shown were transfected in the presence of endogenous Smo. The results of a representative ptc-Luc reporter assay are shown. (B) Overexpression of GFP-tagged Smo-Glu in embryos leads to expansion of Wg expression. Immunofluorescence using anti-GFP (green) and anti-Wg (red) Abs is shown for dorsal views at ×10 and ×25 of embryos at extended germ-band stage that are either wild-type (WT; w1118) or expressing UAS GFP-tagged wild-type (Smo), III,V,VI Ala (Ala), or III,V,VI Glu (Glu) forms of Smo using the prd-GAL4 driver. P ↔ A, orientation of the posterior/anterior axis. (C) Overexpression of Smo variants leads to wing patterning alterations. Wings collected from adult flies that are either wild-type (WT) or heterozygous for the ptc-GAL4 (Upper) or 71B GAL4 (Lower) driver and expressing GFP-Smo variants as in B are shown. Longitudinal veins 1–5 are labeled. Asterisk, proximal L3 and L4 fusion for the GFP-Smo-Ala variant overexpressed with ptc-GAL4; arrowheads, ectopic veination near the proximal end of L3 when GFP-Smo is overexpressed (black) and proximal L3 and L4 fusion when GFP-Smo-Ala is overexpressed (white) using 71B GAL4. Flies expressing GFP-Smo-Glu die as pupae with ptc-GAL4 and as pharate adults with 71B GAL4. A severely defective wing from an escaper from the GFP-Smo-Glu 71B GAL4 line is shown (Lower Right).

Article Snippet: GAL4 driver lines used were prd-GAL4, ptc-GAL4 , and 71B ( 51 ). pUAST-derived plasmids (with the mini white gene) containing GFP-smo coding sequences were injected into w 1118 fly embryos by Genetic Services, Inc. (Sudbury, MA).

Techniques: In Vitro, In Vivo, Activity Assay, Expressing, Transfection, Reporter Assay, Over Expression, Immunofluorescence, Labeling, Variant Assay